Comprehensive transcriptional analysis reveals salt stress-regulated key pathways, hub genes and time-specific responsive gene categories in common bermudagrass (Cynodon dactylon (L.) Pers.) roots

Abstract Background Despite its good salt-tolerance level, key genes and pathways involved with temporal salt response of common bermudagrass ( Cynodon dactylon (L.) Pers.) have not been explored. Therefore, in this study, to understand the underlying regulatory mechanism following different period exposure, a comprehensive transcriptome analysis roots was conducted. Results The transcripts regulated after 1 h, 6 or 24 h hydroponic exposure 200 mM NaCl were investigated. Dataset series revealed 16 distinct salt-responsive expression profiles. Enrichment identified potentially important responsive belonging specific categories, such as hormonal metabolism, secondary misc., cell wall, transcription factors encoded transporters. Weighted gene co-expression network (WGCNA) that lavenderblush2 brown4 modules significantly positively correlated proline content peroxidase activity hub within these two further determined. Besides, treatment, categories signalling receptor kinase, factors, tetrapyrrole synthesis lipid metabolism immediately exclusively up-enriched compared subsequent time points, which indicated fast-acting immediate physiological responses. Genes metabolite biosynthesis simple phenols, glucosinolates, isoflavones tocopherol up-regulated suggesting slightly slower reaction metabolic adjustment. Conclusion Here, we commonly differentially expressed short-term stress, possible adaptive mechanisms roots. Also, distinctive salt-response potential salt-tolerant investigated can provide useful future references explore molecular bermudagrass.